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aav itr u6 sgrna backbone pcbhcre wpre hghpa itr addgene  (Addgene inc)


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    Structured Review

    Addgene inc aav itr u6 sgrna backbone pcbhcre wpre hghpa itr addgene
    Aav Itr U6 Sgrna Backbone Pcbhcre Wpre Hghpa Itr Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+plasmid+60229/AAV%3AITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR+(Plasmid+%2360229)/pm41417735-190-68-69
    Average 94 stars, based on 52 article reviews
    aav itr u6 sgrna backbone pcbhcre wpre hghpa itr addgene - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    In Vivo:

    Article Title: Hedgehog-interacting protein acts in the habenula to regulate nicotine intake.
    Article Snippet: Plasmid and packaged lentivirus particles containing the pGreenFire1-Gli (EF1α-neo) Lentivector reporter were purchased from System Biosciences (catalog nos. .. TR050pa-N and TR050VA-N, respectively). pcDNA-hmmPtch1-B-V111FL114FI766F-HA-TagBFP and pcDNA-h-mmPtch1-B-NNQ-HA-GFP were gifts from Philip Beachy (Stanford University School of Medicine, Stanford, CA 94305; Addgene plasmids 121164 and 120911, respectively). sgRNAs for in vivo CRISPR experiments were cloned into a modified version of Addgene plasmid 60229 donated by the Zhang laboratory: AAV:ITR-U6-sgRNA(backbone)-pCBh-CreWPRE-hGHpA-ITR in which the Cre recombinase cassette was removed. .. The sgRNA of interest (HHIP or eGFP) was cloned in to an AAV1 serotype by Vector Biolabs.

    CRISPR:

    Article Title: Hedgehog-interacting protein acts in the habenula to regulate nicotine intake.
    Article Snippet: Plasmid and packaged lentivirus particles containing the pGreenFire1-Gli (EF1α-neo) Lentivector reporter were purchased from System Biosciences (catalog nos. .. TR050pa-N and TR050VA-N, respectively). pcDNA-hmmPtch1-B-V111FL114FI766F-HA-TagBFP and pcDNA-h-mmPtch1-B-NNQ-HA-GFP were gifts from Philip Beachy (Stanford University School of Medicine, Stanford, CA 94305; Addgene plasmids 121164 and 120911, respectively). sgRNAs for in vivo CRISPR experiments were cloned into a modified version of Addgene plasmid 60229 donated by the Zhang laboratory: AAV:ITR-U6-sgRNA(backbone)-pCBh-CreWPRE-hGHpA-ITR in which the Cre recombinase cassette was removed. .. The sgRNA of interest (HHIP or eGFP) was cloned in to an AAV1 serotype by Vector Biolabs.

    Clone Assay:

    Article Title: Hedgehog-interacting protein acts in the habenula to regulate nicotine intake.
    Article Snippet: Plasmid and packaged lentivirus particles containing the pGreenFire1-Gli (EF1α-neo) Lentivector reporter were purchased from System Biosciences (catalog nos. .. TR050pa-N and TR050VA-N, respectively). pcDNA-hmmPtch1-B-V111FL114FI766F-HA-TagBFP and pcDNA-h-mmPtch1-B-NNQ-HA-GFP were gifts from Philip Beachy (Stanford University School of Medicine, Stanford, CA 94305; Addgene plasmids 121164 and 120911, respectively). sgRNAs for in vivo CRISPR experiments were cloned into a modified version of Addgene plasmid 60229 donated by the Zhang laboratory: AAV:ITR-U6-sgRNA(backbone)-pCBh-CreWPRE-hGHpA-ITR in which the Cre recombinase cassette was removed. .. The sgRNA of interest (HHIP or eGFP) was cloned in to an AAV1 serotype by Vector Biolabs.

    Modification:

    Article Title: Hedgehog-interacting protein acts in the habenula to regulate nicotine intake.
    Article Snippet: Plasmid and packaged lentivirus particles containing the pGreenFire1-Gli (EF1α-neo) Lentivector reporter were purchased from System Biosciences (catalog nos. .. TR050pa-N and TR050VA-N, respectively). pcDNA-hmmPtch1-B-V111FL114FI766F-HA-TagBFP and pcDNA-h-mmPtch1-B-NNQ-HA-GFP were gifts from Philip Beachy (Stanford University School of Medicine, Stanford, CA 94305; Addgene plasmids 121164 and 120911, respectively). sgRNAs for in vivo CRISPR experiments were cloned into a modified version of Addgene plasmid 60229 donated by the Zhang laboratory: AAV:ITR-U6-sgRNA(backbone)-pCBh-CreWPRE-hGHpA-ITR in which the Cre recombinase cassette was removed. .. The sgRNA of interest (HHIP or eGFP) was cloned in to an AAV1 serotype by Vector Biolabs.

    Plasmid Preparation:

    Article Title: Hedgehog-interacting protein acts in the habenula to regulate nicotine intake.
    Article Snippet: Plasmid and packaged lentivirus particles containing the pGreenFire1-Gli (EF1α-neo) Lentivector reporter were purchased from System Biosciences (catalog nos. .. TR050pa-N and TR050VA-N, respectively). pcDNA-hmmPtch1-B-V111FL114FI766F-HA-TagBFP and pcDNA-h-mmPtch1-B-NNQ-HA-GFP were gifts from Philip Beachy (Stanford University School of Medicine, Stanford, CA 94305; Addgene plasmids 121164 and 120911, respectively). sgRNAs for in vivo CRISPR experiments were cloned into a modified version of Addgene plasmid 60229 donated by the Zhang laboratory: AAV:ITR-U6-sgRNA(backbone)-pCBh-CreWPRE-hGHpA-ITR in which the Cre recombinase cassette was removed. .. The sgRNA of interest (HHIP or eGFP) was cloned in to an AAV1 serotype by Vector Biolabs.

    Bioprocessing:

    Article Title: Hedgehog-interacting protein acts in the habenula to regulate nicotine intake.
    Article Snippet: Plasmid and packaged lentivirus particles containing the pGreenFire1-Gli (EF1α-neo) Lentivector reporter were purchased from System Biosciences (catalog nos. .. TR050pa-N and TR050VA-N, respectively). pcDNA-hmmPtch1-B-V111FL114FI766F-HA-TagBFP and pcDNA-h-mmPtch1-B-NNQ-HA-GFP were gifts from Philip Beachy (Stanford University School of Medicine, Stanford, CA 94305; Addgene plasmids 121164 and 120911, respectively). sgRNAs for in vivo CRISPR experiments were cloned into a modified version of Addgene plasmid 60229 donated by the Zhang laboratory: AAV:ITR-U6-sgRNA(backbone)-pCBh-CreWPRE-hGHpA-ITR in which the Cre recombinase cassette was removed. .. The sgRNA of interest (HHIP or eGFP) was cloned in to an AAV1 serotype by Vector Biolabs.



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